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mirna microarray chip μparaflo microfluidic chip  (LC Sciences)

 
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    LC Sciences mirna microarray chip μparaflo microfluidic chip
    The significantly differentially expressed miRNAs in aging kidney
    Mirna Microarray Chip μparaflo Microfluidic Chip, supplied by LC Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mirna+microarray+chip+%CE%BCparaflo+microfluidic+chip/pmc03137573-246-6-0?v=LC+Sciences
    Average 90 stars, based on 1 article reviews
    mirna microarray chip μparaflo microfluidic chip - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "miR-335 and miR-34a Promote Renal Senescence by Suppressing Mitochondrial Antioxidative Enzymes"

    Article Title: miR-335 and miR-34a Promote Renal Senescence by Suppressing Mitochondrial Antioxidative Enzymes

    Journal: Journal of the American Society of Nephrology : JASN

    doi: 10.1681/ASN.2010040367

    The significantly differentially expressed miRNAs in aging kidney
    Figure Legend Snippet: The significantly differentially expressed miRNAs in aging kidney

    Techniques Used:

    miR-184, miR-335 and miR-347 exhibited differential expressions in (A) aged rats and (B) mice. (A) Fold differences between old and young rat kidneys measured by chip and qRT-PCR. (B) Expression levels of mmu-miR-184, mmu-miR-335, and mmu-miR-347 in the aged mouse kidneys detected by qRT-PCR. n = 5 per miRNA. *P < 0.01 versus young.
    Figure Legend Snippet: miR-184, miR-335 and miR-347 exhibited differential expressions in (A) aged rats and (B) mice. (A) Fold differences between old and young rat kidneys measured by chip and qRT-PCR. (B) Expression levels of mmu-miR-184, mmu-miR-335, and mmu-miR-347 in the aged mouse kidneys detected by qRT-PCR. n = 5 per miRNA. *P < 0.01 versus young.

    Techniques Used: Quantitative RT-PCR, Expressing

    Antioxidation-related miRNAs differentially expressed in old kidney and their target genes
    Figure Legend Snippet: Antioxidation-related miRNAs differentially expressed in old kidney and their target genes

    Techniques Used:

    Expressions of miR-335 and miR-34a in aging mesangial cells, endothelial cells, tubular epithelial cells and interstitial fibroblasts were significantly upregulated and expressions of SOD2 and Txnrd2 in aging mesangial cells were downregulated. (A) Expression levels of miR-335 and miR-34a in aging renal mesangial cells were detected by qRT-PCR. n = 5 per miRNA. *P < 0.01 versus young. (B) Levels of SOD2 and Txnrd2 proteins were analyzed by Western blot in aging renal mesangial cells. The graph is representative of three separate experiments. (C) Expression levels of miR-335 and miR-34a were detected by qRT-PCR in the aging glomerular epithelial cells, endothelial cells, tubular epithelial cells, and fibroblasts. n = 5 per miRNA. *P < 0.01 versus young, #P < 0.05 versus young.
    Figure Legend Snippet: Expressions of miR-335 and miR-34a in aging mesangial cells, endothelial cells, tubular epithelial cells and interstitial fibroblasts were significantly upregulated and expressions of SOD2 and Txnrd2 in aging mesangial cells were downregulated. (A) Expression levels of miR-335 and miR-34a in aging renal mesangial cells were detected by qRT-PCR. n = 5 per miRNA. *P < 0.01 versus young. (B) Levels of SOD2 and Txnrd2 proteins were analyzed by Western blot in aging renal mesangial cells. The graph is representative of three separate experiments. (C) Expression levels of miR-335 and miR-34a were detected by qRT-PCR in the aging glomerular epithelial cells, endothelial cells, tubular epithelial cells, and fibroblasts. n = 5 per miRNA. *P < 0.01 versus young, #P < 0.05 versus young.

    Techniques Used: Expressing, Quantitative RT-PCR, Western Blot

    (A) miR-335 and miR-34a mimics inhibit SOD2 and Txnrd2 expressions in young mesangial cells, and (B) antisense miR-335 and miR-34a increase SOD2 and Txnrd2 expressions in aging mesangial cells. Co, miRNA control; CA, antisense miRNA control; miR335 and miR34a, miR-335 and miR-34a mimics, respectively; AmiR335 and AmiR34a, antisense miR-335 and miR-34a inhibitors, respectively. (C) Analysis of expression levels of SOD2 and Txnrd2 by Western blot in aged renal tissues. The graph is representative of three separate experiments.
    Figure Legend Snippet: (A) miR-335 and miR-34a mimics inhibit SOD2 and Txnrd2 expressions in young mesangial cells, and (B) antisense miR-335 and miR-34a increase SOD2 and Txnrd2 expressions in aging mesangial cells. Co, miRNA control; CA, antisense miRNA control; miR335 and miR34a, miR-335 and miR-34a mimics, respectively; AmiR335 and AmiR34a, antisense miR-335 and miR-34a inhibitors, respectively. (C) Analysis of expression levels of SOD2 and Txnrd2 by Western blot in aged renal tissues. The graph is representative of three separate experiments.

    Techniques Used: Control, Expressing, Western Blot

    miR-335 and miR-34a mimics induce premature senescent phenotypes in young mesangial cells, and antisense miR-335 and miR-34a relieve senescent phenotypes in old mesangial cells. (A) SA-β-gal staining in young mesangial cells transfected with miR-335 and miR-34a mimics. Blue precipitation in the cytoplasm was observed in the senescent cells. (B) Analysis of SAHF formation in young mesangial cells transfected with miR-335 and miR-34a mimics. The cells were stained with DAPI, and heterochromatin foci were observed in the senescent cells. (C) SA-β-gal staining in aging mesangial cells transfected with antisense miR-335 and miR-34a inhibitors. (D) Analysis of SAHF formation in aging mesangial cells transfected with antisense miR-335 and miR-34a inhibitors. Co, miRNA control; CA, antisense miRNA control. The above results of SA-β-gal staining and SAHF analysis are representative images of three experiments.
    Figure Legend Snippet: miR-335 and miR-34a mimics induce premature senescent phenotypes in young mesangial cells, and antisense miR-335 and miR-34a relieve senescent phenotypes in old mesangial cells. (A) SA-β-gal staining in young mesangial cells transfected with miR-335 and miR-34a mimics. Blue precipitation in the cytoplasm was observed in the senescent cells. (B) Analysis of SAHF formation in young mesangial cells transfected with miR-335 and miR-34a mimics. The cells were stained with DAPI, and heterochromatin foci were observed in the senescent cells. (C) SA-β-gal staining in aging mesangial cells transfected with antisense miR-335 and miR-34a inhibitors. (D) Analysis of SAHF formation in aging mesangial cells transfected with antisense miR-335 and miR-34a inhibitors. Co, miRNA control; CA, antisense miRNA control. The above results of SA-β-gal staining and SAHF analysis are representative images of three experiments.

    Techniques Used: Staining, Transfection, Control



    Similar Products

    mirna microarray chip μparaflo microfluidic chip  (LC Sciences)
    90
    LC Sciences mirna microarray chip μparaflo microfluidic chip
    The significantly differentially expressed miRNAs in aging kidney
    Mirna Microarray Chip μparaflo Microfluidic Chip, supplied by LC Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mirna+microarray+chip+%CE%BCparaflo+microfluidic+chip/pmc03137573-246-6-0?v=LC+Sciences
    Average 90 stars, based on 1 article reviews
    mirna microarray chip μparaflo microfluidic chip - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    The significantly differentially expressed miRNAs in aging kidney

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: miR-335 and miR-34a Promote Renal Senescence by Suppressing Mitochondrial Antioxidative Enzymes

    doi: 10.1681/ASN.2010040367

    Figure Lengend Snippet: The significantly differentially expressed miRNAs in aging kidney

    Article Snippet: LC Sciences (Houston, TX) provided the miRNA microarray chip (μParaflo microfluidic chip).

    Techniques:

    miR-184, miR-335 and miR-347 exhibited differential expressions in (A) aged rats and (B) mice. (A) Fold differences between old and young rat kidneys measured by chip and qRT-PCR. (B) Expression levels of mmu-miR-184, mmu-miR-335, and mmu-miR-347 in the aged mouse kidneys detected by qRT-PCR. n = 5 per miRNA. *P < 0.01 versus young.

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: miR-335 and miR-34a Promote Renal Senescence by Suppressing Mitochondrial Antioxidative Enzymes

    doi: 10.1681/ASN.2010040367

    Figure Lengend Snippet: miR-184, miR-335 and miR-347 exhibited differential expressions in (A) aged rats and (B) mice. (A) Fold differences between old and young rat kidneys measured by chip and qRT-PCR. (B) Expression levels of mmu-miR-184, mmu-miR-335, and mmu-miR-347 in the aged mouse kidneys detected by qRT-PCR. n = 5 per miRNA. *P < 0.01 versus young.

    Article Snippet: LC Sciences (Houston, TX) provided the miRNA microarray chip (μParaflo microfluidic chip).

    Techniques: Quantitative RT-PCR, Expressing

    Antioxidation-related miRNAs differentially expressed in old kidney and their target genes

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: miR-335 and miR-34a Promote Renal Senescence by Suppressing Mitochondrial Antioxidative Enzymes

    doi: 10.1681/ASN.2010040367

    Figure Lengend Snippet: Antioxidation-related miRNAs differentially expressed in old kidney and their target genes

    Article Snippet: LC Sciences (Houston, TX) provided the miRNA microarray chip (μParaflo microfluidic chip).

    Techniques:

    Expressions of miR-335 and miR-34a in aging mesangial cells, endothelial cells, tubular epithelial cells and interstitial fibroblasts were significantly upregulated and expressions of SOD2 and Txnrd2 in aging mesangial cells were downregulated. (A) Expression levels of miR-335 and miR-34a in aging renal mesangial cells were detected by qRT-PCR. n = 5 per miRNA. *P < 0.01 versus young. (B) Levels of SOD2 and Txnrd2 proteins were analyzed by Western blot in aging renal mesangial cells. The graph is representative of three separate experiments. (C) Expression levels of miR-335 and miR-34a were detected by qRT-PCR in the aging glomerular epithelial cells, endothelial cells, tubular epithelial cells, and fibroblasts. n = 5 per miRNA. *P < 0.01 versus young, #P < 0.05 versus young.

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: miR-335 and miR-34a Promote Renal Senescence by Suppressing Mitochondrial Antioxidative Enzymes

    doi: 10.1681/ASN.2010040367

    Figure Lengend Snippet: Expressions of miR-335 and miR-34a in aging mesangial cells, endothelial cells, tubular epithelial cells and interstitial fibroblasts were significantly upregulated and expressions of SOD2 and Txnrd2 in aging mesangial cells were downregulated. (A) Expression levels of miR-335 and miR-34a in aging renal mesangial cells were detected by qRT-PCR. n = 5 per miRNA. *P < 0.01 versus young. (B) Levels of SOD2 and Txnrd2 proteins were analyzed by Western blot in aging renal mesangial cells. The graph is representative of three separate experiments. (C) Expression levels of miR-335 and miR-34a were detected by qRT-PCR in the aging glomerular epithelial cells, endothelial cells, tubular epithelial cells, and fibroblasts. n = 5 per miRNA. *P < 0.01 versus young, #P < 0.05 versus young.

    Article Snippet: LC Sciences (Houston, TX) provided the miRNA microarray chip (μParaflo microfluidic chip).

    Techniques: Expressing, Quantitative RT-PCR, Western Blot

    (A) miR-335 and miR-34a mimics inhibit SOD2 and Txnrd2 expressions in young mesangial cells, and (B) antisense miR-335 and miR-34a increase SOD2 and Txnrd2 expressions in aging mesangial cells. Co, miRNA control; CA, antisense miRNA control; miR335 and miR34a, miR-335 and miR-34a mimics, respectively; AmiR335 and AmiR34a, antisense miR-335 and miR-34a inhibitors, respectively. (C) Analysis of expression levels of SOD2 and Txnrd2 by Western blot in aged renal tissues. The graph is representative of three separate experiments.

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: miR-335 and miR-34a Promote Renal Senescence by Suppressing Mitochondrial Antioxidative Enzymes

    doi: 10.1681/ASN.2010040367

    Figure Lengend Snippet: (A) miR-335 and miR-34a mimics inhibit SOD2 and Txnrd2 expressions in young mesangial cells, and (B) antisense miR-335 and miR-34a increase SOD2 and Txnrd2 expressions in aging mesangial cells. Co, miRNA control; CA, antisense miRNA control; miR335 and miR34a, miR-335 and miR-34a mimics, respectively; AmiR335 and AmiR34a, antisense miR-335 and miR-34a inhibitors, respectively. (C) Analysis of expression levels of SOD2 and Txnrd2 by Western blot in aged renal tissues. The graph is representative of three separate experiments.

    Article Snippet: LC Sciences (Houston, TX) provided the miRNA microarray chip (μParaflo microfluidic chip).

    Techniques: Control, Expressing, Western Blot

    miR-335 and miR-34a mimics induce premature senescent phenotypes in young mesangial cells, and antisense miR-335 and miR-34a relieve senescent phenotypes in old mesangial cells. (A) SA-β-gal staining in young mesangial cells transfected with miR-335 and miR-34a mimics. Blue precipitation in the cytoplasm was observed in the senescent cells. (B) Analysis of SAHF formation in young mesangial cells transfected with miR-335 and miR-34a mimics. The cells were stained with DAPI, and heterochromatin foci were observed in the senescent cells. (C) SA-β-gal staining in aging mesangial cells transfected with antisense miR-335 and miR-34a inhibitors. (D) Analysis of SAHF formation in aging mesangial cells transfected with antisense miR-335 and miR-34a inhibitors. Co, miRNA control; CA, antisense miRNA control. The above results of SA-β-gal staining and SAHF analysis are representative images of three experiments.

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: miR-335 and miR-34a Promote Renal Senescence by Suppressing Mitochondrial Antioxidative Enzymes

    doi: 10.1681/ASN.2010040367

    Figure Lengend Snippet: miR-335 and miR-34a mimics induce premature senescent phenotypes in young mesangial cells, and antisense miR-335 and miR-34a relieve senescent phenotypes in old mesangial cells. (A) SA-β-gal staining in young mesangial cells transfected with miR-335 and miR-34a mimics. Blue precipitation in the cytoplasm was observed in the senescent cells. (B) Analysis of SAHF formation in young mesangial cells transfected with miR-335 and miR-34a mimics. The cells were stained with DAPI, and heterochromatin foci were observed in the senescent cells. (C) SA-β-gal staining in aging mesangial cells transfected with antisense miR-335 and miR-34a inhibitors. (D) Analysis of SAHF formation in aging mesangial cells transfected with antisense miR-335 and miR-34a inhibitors. Co, miRNA control; CA, antisense miRNA control. The above results of SA-β-gal staining and SAHF analysis are representative images of three experiments.

    Article Snippet: LC Sciences (Houston, TX) provided the miRNA microarray chip (μParaflo microfluidic chip).

    Techniques: Staining, Transfection, Control